University of Pittsburgh PITT HOME  |  FIND PEOPLE  |  CONTACT US
  Pittsburgh Molecular Libraries Screening Center
HOME
HISTORY
SCREENING TECHNOLOGY
COMPOUND LIBRARIES
INSTRUMENTATION/PLATFORMS
HTS GUIDELINES
APPROVED PMLSC ASSAY PROTOCOLS
  • PMLSC Assay Protocol Review Document for MKP-1 HTS
  • PMLSC Assay Protocol Review Document for Cdc25B Catalytic Domain HTS
  • GNHR Translocation Assay APAC Review document
  • Necroptosis PMLSC APAC Document
  • PLK-1 PBD PMLSC APAC Document
  • West Nile Virus NS2bNS3 PMLSC APAC Document
  • Time Resolved Fluorescence Resonance Energy Transfer assay for Plk1 inhibitors
  • PKD IMAP TR-FRET APAC Document
  • High-content cell-based screening for modulators of autophagy
  • MKP-3 Chemical Complementation assay
  • PMLSC PROBE REPORTS
    DATA ANALYSIS/INFORMATICS
    PUBLICATIONS
    LINKS
    CONTACTS
    Approved PMLSC Assay Protocols
     
    bullet point  PMLSC Assay Protocol Review Document for Cdc25B Catalytic Domain HTS
     
     
    Cdc25 is a dual specificity protein tyrosine phosphatase that plays a pivotal role in the regulation of the cell cycle. Of the three isoforms that exist (Cdc25A, B, and C), Cdc25A and Cdc25B have been found to be oncongenic and over-expressed in many cancer cell lines. Cdc25B has been a target in multiple drug discovery endeavors, leading to several inhibitors of phosphatase activity in the micromolar to sub-micromolar range. Many of these compounds have been shown to induce G2/M cell cycle arrest with anti-proliferative effects. The Cdc25B catalytic domain assay is a homogeneous fluorescence intensity assay measuring the hydrolysis of the generic substrate 3-O-methylfluorescein phosphate (OMFP). We describe here the optimization that was required for the development of a 384-well HTS assay that conforms to the PMLSC assay development and implementation guidelines.
     
    Documents
  • PMLSC Assay Protocol Review Document for Cdc25B Catalytic Domain HTS
  •  
      to topto top
     
     
       Health Sciences @ Pitt   |    UPMC   |    HSLS   |    School of Medicine   |    Health Sciences Calendar   |    Our News & Events  
    Top of Page  |  Home  |  Contact Us Last Update 4/29/2008
    Office of the Senior Vice Chancellor for Health Sciences, University of Pittsburgh. All rights reserved.