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  • PMLSC Assay Protocol Review Document for MKP-1 HTS
  • PMLSC Assay Protocol Review Document for Cdc25B Catalytic Domain HTS
  • GNHR Translocation Assay APAC Review document
  • Necroptosis PMLSC APAC Document
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  • PKD IMAP TR-FRET APAC Document
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  • MKP-3 Chemical Complementation assay
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    Approved PMLSC Assay Protocols
     
    bullet point  GNHR Translocation Assay APAC Review document
     
     
    Cytoplasmic dynein is the molecular motor that carries cargo to the minus ends of microtubules (MTs) (e.g., from the cytoplasm to the nucleus), and provides the mechancial force for many other important fuctions, including nuclear envelope breakdown and sister chromatid exchange at mitosis. Unlike the numerous MT plus end-directed molecular motors, the kinesins, no specific small molecule inhibitors of dynein are known. Weak and nonspecific redox perturbers and nonspecific mimics of ATP (e.g., e/ytf""o-9-hydroxynonyladenine, aka EHNA) or phosphate anion
    (e.g., vanadate) are the only known dynein inhibitors. New inhibitors with potency and specificity would be invaluable cell biology tools. This void in our chemical biology toolbox is due in large part to both the large size of the work-performing component of the complex, dynein heavy chain (DYNC1H1) and the working complex itself, as well as the relative difficulty of working with functional dynein from biological systems. The overall goal of this work is to develop a refined suite of high throughput cell and biochemical assays for screening of chemical libraries to find experimentally useful inhibitors of cellular cytoplasmic dynein.
     
    Documents
  • PMLSC APAC Reports\GNHR Translocation Assay APAC Review document
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