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APPROVED PMLSC ASSAY PROTOCOLS
  • PMLSC Assay Protocol Review Document for MKP-1 HTS
  • PMLSC Assay Protocol Review Document for Cdc25B Catalytic Domain HTS
  • GNHR Translocation Assay APAC Review document
  • Necroptosis PMLSC APAC Document
  • PLK-1 PBD PMLSC APAC Document
  • West Nile Virus NS2bNS3 PMLSC APAC Document
  • Time Resolved Fluorescence Resonance Energy Transfer assay for Plk1 inhibitors
  • PKD IMAP TR-FRET APAC Document
  • High-content cell-based screening for modulators of autophagy
  • MKP-3 Chemical Complementation assay
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    Approved PMLSC Assay Protocols
     
    bullet point  PLK-1 PBD PMLSC APAC Document
     
     
    The Polo-like kinases (Plks) play important roles in many cell cycle-related events including the initiation of mitosis, chromosome segregation, centrosome maturation, bipolar spindle formation, regulation of anaphase-promoting complex and execution of cytokinesis. The prototypic Polo kinase was originally identified in flies, in which mutants resulted in abnormal spindle poles. A single Polo family member is found in flies, budding yeast, and fission yeast (Polo, CDC5 and Plo1, respectively), while 3 Plk family members are found in humans, mice and frogs (Plk1/Plx1, Plk2/Snk/Plx2 and Plk3/Fnk/Prk/Plx3 respectively). One human family member, Plk1, as well as its homologues in Xenopus (Plx1) and budding yeast (Cdc5) plays a particularly important role in coordinating multiple events during mitosis. Plk1/Plx1/Cdc5 is critical for proper centrosome maturation and spindle assembly. Plk1/Plx1/Cdc5 protein expression peaks during late G2 and M phases, where it localizes to centrosomes and spindle pole bodies during prophase and metaphase. During this time, Plk1/Plx1 likely phosphorylates a number of key centrosome-associated proteins, including the mitotic phosphatase Cdc25C to initiate the Cdc25C-Cdc2 amplification loop at the onset of M phase. At metaphase, Plk1 activates the anaphase-promoting complex (APC) to upregulate the ubiquitin-dependent proteolytic degradation of factors that regulate passage through mitosis. In addition, Plk1 and its homologues play important roles in the later stages of mitosis, particularly in anaphase and cytokinesis. In budding yeast, for example, Cdc5 phosphorylates the Scc1 subunit of the cohesin complex, which holds sister chromatids together until the start of anaphase. Phosphorylation of Scc1 by Cdc5 facilitates proteolytic cleavage of Scc1 by separase to allow sister chromatid separation upon anaphase onset. Plk1 re-localizes to the spindle midzone during late anaphase, possibly playing roles in regulating components of microtubules and kinetochores, and subsequently re-localizes to the mid-body during telophase and cytokinesis, where it appears to play a critical role in formation of the contractile ring at the site of cytoplasmic division.
     
    Documents
  • PLK-1 PBD PMLSC APAC Document
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