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APPROVED PMLSC ASSAY PROTOCOLS
  • PMLSC Assay Protocol Review Document for MKP-1 HTS
  • PMLSC Assay Protocol Review Document for Cdc25B Catalytic Domain HTS
  • GNHR Translocation Assay APAC Review document
  • Necroptosis PMLSC APAC Document
  • PLK-1 PBD PMLSC APAC Document
  • West Nile Virus NS2bNS3 PMLSC APAC Document
  • Time Resolved Fluorescence Resonance Energy Transfer assay for Plk1 inhibitors
  • PKD IMAP TR-FRET APAC Document
  • High-content cell-based screening for modulators of autophagy
  • MKP-3 Chemical Complementation assay
  • PMLSC PROBE REPORTS
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    Approved PMLSC Assay Protocols
     
    bullet point  High-content cell-based screening for modulators of autophagy
     
     
    The High-content cell-based assay for screening modulators of autophagy is the resultof a joint development project between Xiao-Ming Yin at the Department ofPharmacology and Andreas Vogt, UPDDI. The assay was jointly developed andoptimized and submitted to the NIH in response to PAR -06-545 (PI : Yin). Theapplication (1R03 MH-083154-01) received a priority score of 159 (16.9th percentile) on
    August 10, 2007 and was awarded to Dr. Yin on September 26, 2007. The applicationrequested to be assigned to the PMLSC. The NIH has extended a request to file an MTA to Dr. Yin the first week of September, 2007.
    We report here the assay optimization that was required to evaluate the suitability for HTS of the High-content cell-based screening for modulators of autophagy according to
    UPMLSC assay development and implementation guidelines.
     
    Documents
  • HCS for Autophagy APAC Document
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